Tuesday: Competitive Inhibition and General Acid/Base Catalysis
Goals of the day - Mechanism of competitive inhibition. Enthalpy & Entropy in catalysis. Catalysis by strain. Entropy Traps. Drawing enzyme mechanisms with GA/GB catalysis. Activity-pH profiles. Triose phosphate isomerase. pKa tuning by environment.

• My lecture notes on general acid/base catalysis
• My lecture notes on triose phosphate isomerase
• My powerpoint slides
• Additional Reading
• Two reviews on Triose Phosphate Isomerase from Jeremy Knowles: Not Different, Just Better and To Build an Enzyme

Thursday: General Acid/Base continued
Goals of the day - Structure and Mechanism in triose phosphate isomerase. RNase A - mechanisms, structure, bronsted analysis.

• My notes on catalysis of RNA hydrolysis
• Read V&V Ch. 15.3 or G&G Ch. 14
• My powerpoint slides
• Additional Reading
• Good review by Ron Raines on RNase A.
• Article by Ron Raines on GA/GB catalysis by RNase A.