Tuesday: Amino acyl tRNA synthetases
Goals of the day - Specificity in catalysis - with respect to amino acid and tRNA substrates. Hopfield Mechanism
- My notes on amino acyl tRNA synthtases.
- My notes on the Hopfield Mechanism.
- JJ Hopfield (1974) Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity. PNAS, 71, 4135-4139.
- My powerpoint slides
- Additional Reading
- The structure of AlaRS bound to a cognate and non-cognate tRNA. Also a nice quick review.
Thursday: Peptide synthesis and the ribosome
Goals of the day - On to the ribosome and translation. tRNA structure/function. Specificity in codon:anticodon recognition